The polymerase chain reaction (PCR) is a process that amplifies a single or a few copies of a piece of DNA generating thousands to millions of copies of a particular DNA sequence.
Vector Cloning involves vectors (vehicles by which DNA may be introduced into host cells by) that are introduced into host cells. Fragments of the DNA introduced into the cell are identically cloned when the cell replicates and divides.
SIMILARITIES:
- Produce a sequence of DNA in large quantities
- DNA is produced at a fast rate
- Use electrophoresis
DIFFERENCES:
- PCR uses DNA primers, Taq polymerase, and dNTPS
- Vector cloning requires a plasmid vector and a bacterial host
- PCR produces a pure sample of the DNA of interest
- Vector cloning expresses the gene (the gene of interest that was cloned can produce proteins)
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